Hippocampal Plasticity – An Update

Posted in Clinical Review Article on 19th Jun 2013

Author

Olivia Shipton & Ole Paulsen

Olivia Shipton is a final-year DPhil student on the Wellcome Trust-funded Oxford Ion Channels and Disease Initiative (OXION). She is interested in the role of hippocampal plasticity in learning and memory, and its impairment in Alzheimer's disease.

Ole Paulsen MD/PhD is the Professor of Physiology (1883) at the University of Cambridge. He is interested in the relationships between network architecture, circuit dynamics and synaptic plasticity in health and disease.

Correspondence to:
Professor Ole Paulsen
Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge CB2 3EG, UK.
E-mail: Professor Ole Paulson

Review-Shipton-picIt is forty years since the first full report of long-term potentiation (LTP), a presumed cellular substrate for learning and memory. This review will provide an update on some recent advances in our understanding of the mechanisms underlying hippocampal LTP and address their potential clinical relevance.

Review-shipton-paulsen

Introduction

The hippocampus plays a fundamental role in learning and memory processes, as famously discovered with the study of patient HM, who lost the ability to lay down new memories after surgical removal of the medial temporal lobes.1 Not only is the hippocampus necessary for memory formation, it is also activated during memory tasks (Figure 1). The acquired information is thought to be stored as changes in the connections between hippocampal neurons and such changes are known as synaptic plasticity, the strengthening and weakening of synaptic efficacy. The best-described hippocampal plasticity process is that of long-term potentiation (LTP), which will be discussed here.

Figure 1.

Figure1: The human hippocampus is activated during a memory task involving word recall. Note the asymmetry, such that only the right hippocampal formation shows activity greater than baseline levels. Reproduced with kind permission of Larry Squire (from ref.28).

Forty years ago, Bliss and Lømo reported that a high-frequency train of presynaptic stimulation in the rabbit hippocampus in vivo caused strength- ening of the postsynaptic response to a given stimulation,2 confirming earlier suggestions about activity-driven synaptic changes proposed independently by Konorski (1948)3 and Hebb (1949)4. This “long-lasting potentiation” is now known as LTP and has received much experimental attention over the years due to the widely held view that it could be a cellular substrate for learning and memory. Initial exploratory research confirmed that LTP was found in other animals, other hippocampal pathways and brain regions, and even in in vitro brain slice preparations,which proved particularly important for further detailed experimental dissection.

Thirty years ago, a fundamental step forward was made in our mechanistic understanding of LTP with the demonstration of NMDA receptor (NMDAR) involvement.5 Upon activation by synaptically-released glutamate,NMDARs open an ion channel permeable to Ca2+, required for LTP.6 Ten years later, Bliss and Collingridge summarised the intracellular pathways of plasticity that had been uncovered so far and outlined a number of crucial unresolved issues in their seminal review.7 So how far have we come in answering them?

Major recent advances

The long-established mechanism of hippocampal LTP involves the presynaptic release of glutamate along with a postsynaptic depolarisation sufficient to relieve the NMDAR of its Mg2+ block.The ensuing Ca2+ influx through NMDARs can then trigger downstream kinase cascades that cause the increased recruitment of AMPA receptors (AMPARs) to the postsynaptic membrane, which supports the initial increase in synaptic strength.In the longer term, changes in synaptic strength are maintained by synaptic remodelling. Significant progress has been made in our understanding of the mechanisms underlying the induction,expression and maintenance of synaptic plasticity.

Induction

The necessity of NMDARs for the induction of hippocampal LTP is unrefuted; indeed,the crucial feature of LTP induction protocols is that they cause sufficient depolarisation to permit a high level of Ca2+ influx through these NMDARs. The induction protocols most commonly used are high-frequency (‘tetanic’) stimulation (Figure 2), theta burst stimulation and pre-postsynaptic spike pairing.The latter paradigm induces spike timing-dependent potentiation (tLTP),8 which confirms Hebb’s prediction that potentiation occurs when the presynaptic neuron takes part in firing the postsynaptic neuron.4 tLTP is, therefore, an attrac- tive model for learning as these precise timing requirements of neuronal activity indicate a mechanism for encoding associations in the external world. NMDARs comprise four subunits, two obligatory GluN1 subunits and two additional GluN2 subunits,of which there are different types. It is increasingly recognised that GluN2B subunit- containing NMDARs are required to support LTP,9 most likely because of the tight association between the GluN2B subunit and Ca2+/calmodulin-dependent protein kinase II, which has been identified as a crucial enzyme in LTP induction.10 Interestingly, GluN2B subunit-containing NMDARs may be particularly vulnerable to, and help mediate, the synaptotoxic effects of amyloid beta,11 and so further understanding the unique role they play at the synapse and in LTP could help with treatment of neurodegenerative diseases that affect learning and memory.

Review-shipton-fig-2

Figure 2: Hippocampal long-term potentiation (LTP). Excitatory postsynaptic potentials (EPSPs) are monitored over a baseline period (top panel, left) and then a specific induction stimulation para- digm is applied (red arrow). Following tetanic stimulation, an immediate post-tetanic potentiation is seen, followed by a long-lasting increase in the magnitude of the EPSP (top panel, right) that represents a strengthening of synaptic efficacy.

Expression

It is well established that a greater number of AMPARs at the postsynaptic membrane supports the increased synaptic strength in LTP. This process involves initial recruitment of GluA1 subunit- containing AMPARs to the postsynaptic membrane and their subsequent replacement by GluA2- containing AMPARs to support a continued enhancement of synaptic strength.12 However, the mechanism has recently emerged as more complex than simply direct insertion of AMPARs at the post- synaptic site, and instead it appears that a three- stage model may explain this increase.13 Firstly, LTP triggers exocytosis of GluA1-containing AMPARs to extra-and peri-synaptic regions; secondly, these diffuse laterally into synaptic sites; thirdly, receptors must be captured by the scaffolding of the postsynaptic density in order to be maintained at the synapse. Although LTP pathways likely exert tight regulation of the initial membrane insertion stage, a recent paper suggests that the subsequent steps of this process may be more flexible. Granger et al found that, although a reserve pool of receptors was required to support synaptic strengthening in the hippocampus, the subtype identity of the AMPARs was unimportant; moreover, even the kainate receptor, a glutamate receptor not normally found at that synapse,could substitute.14 Although this research was conducted with endogenous AMPARs deleted, this newly found flexibility at the synapse has implications for the clinic, since it opens the door to the introduction of new designer receptors to restore synaptic function in neurodegenerative conditions. However, this lack of specificity is a double-edged sword,since perturbing this process at a synapse could have many unforeseen consequences.

Maintenance

It has been suggested that LTP is maintained by persistent kinase activity. A strong candidate for this role appeared to be a kinase known as PKM., since blocking it with a peptide called ZIP reversed both hippocampal LTP and specific spatial memories.15 However, the importance of this ‘memory molecule’ has recently been questioned as two labs have reported that hippocampal LTP and hippocampus-dependent learning are present in both constitutive and conditional PKM. knock-out mice.16-17 In fact,it seems unlikely that a mechanism merely involving elevated kinase activity could maintain information storage over years, and a better candidate to sustain enhanced synaptic strength would be structural changes at the synapse. Imaging in neuronal cultures and in animals in vivo has revealed two major structural changes associated with LTP. Firstly, pre-existing thin postsynaptic spines enlarge.18 Secondly, new spines appear, and LTP also correlates with the stabilisation of such nascent spines.19 When spines enlarge sufficiently they become known as mushroom spines; chronic imaging has revealed that spines of this type can be stable for months20 and are not permanently changed following an LTP protocol,18 suggesting they may be robust to perturbations and hence able to act as a‘memory store’.The positive correlation between the volume of the posterior hippocampi of London taxi drivers and the length of time spent in this hippocampally-demanding career is an indication, although not without alternative interpretations, that long-term structural changes also exist in humans.21

Plasticity and behaviour

A major challenge for the future is to understand the relationship between plasticity and behaviour. Although the implicit and explicit assumption has been that plasticity supports memory,22 we still only have correlative evidence for this assertion, and, combined with the recent finding that NMDARs in the CA1 are not always necessary for hippocampus-dependent learning and memory,23 it remains unresolved. A newly developed technology, known as optogenetics, which allows acute control of neuronal activity in regionally-and genetically-defined cell populations by light,may help probe a causal link between plasticity and memory. This has already been used at the cellular level to show that reactivating neurons in the dentate gyrus of the hippocampus that were active during acquisition of fear conditioning triggers expression of the context-dependent fear memory.24 The next stage is to investigate the relationship between plasticity and behaviour at the level of synapses. One promising model to investigate whether distributed changes in synaptic weights can support learning and memory may be the synapse between the excitatory neurons of the CA3 and CA1 regions in the mouse hippocampus. Here, an unexpected asymmetric distribution of postsynaptic spine types has been found whereby spines in CA1 neurons that receive input from the CA3 of the left hippocampus are mostly small and rich in GluN2B subunit-containing NMDARs, while those receiving right CA3 projections are larger and rich in AMPARs25 (Figure 3). Due to this hemispheric asymmetry, these different types of spines can be targeted optogenetically and it was found that only the GluN2B-rich spines receiving left CA3 input show tLTP.26  It is now possible to manipulate these two types of synapses with their different propensity for plasticity to test whether they have differential effects on learning and memory in behaving mice.

Figure 3. Excitatory neurons of the left and right CA3 region of the hippocampus project both ipsilaterally (Schaffer collaterals) and contralaterally (commisural connections) to pyramidal neurons of the CA1. These excitatory projections from CA3 pyramidal neurons to the CA1 can be selectively targeted by stereotactic injection of an optogenetic construct into the CA3 of one hemisphere (top panel: image depicts a right injection; green: fluorescent tag expressed from the optogenetic construct; blue: nuclear stain). In mice, the nature of the CA1 spine is dependent on the location of the presynaptic CA3 neuron from which it receives its input. CA1 spines receiving input from CA3 neurons in the left hippocampus are small spines that are GluN2B-rich. Conversely, CA1 spines receiving input from CA3 neurons in the right hippocampus are large, mushroom-shaped spines that are rich in AMPA receptors (bottom panel). Only those synapses that receive left CA3 input express tLTP, and this correlates with the distribution of GluN2B subunit-containing NMDA receptors.

Figure 3. Excitatory neurons of the left and right CA3 region of the hippocampus project both ipsilaterally (Schaffer collaterals) and contralaterally (commisural connections) to pyramidal neurons of the CA1. These excitatory projections from CA3 pyramidal neurons to the CA1 can be selectively targeted by stereotactic injection of an optogenetic construct into the CA3 of one hemisphere (top panel: image depicts a right injection; green: fluorescent tag expressed from the optogenetic construct; blue: nuclear stain). In mice, the nature of the CA1 spine is dependent on the location of the presynaptic CA3 neuron from which it receives its input. CA1 spines receiving input from CA3 neurons in the left hippocampus are small spines that are GluN2B-rich. Conversely, CA1 spines receiving input from CA3 neurons in the right hippocampus are large, mushroom-shaped spines that are rich in AMPA receptors (bottom panel). Only those synapses that receive left CA3 input express tLTP, and this correlates with the distribution of GluN2B subunit-containing NMDA receptors.

Clinical relevance

Synaptic dysfunction is a central component of a wide range of neurological conditions. Understanding the basic mechanisms involved in hippocampal LTP may make it possible to alter plasticity and neural networks in predictable ways, and the hope is that this could be used therapeutically in the future. For example, the early stages of Alzheimer’s disease are characterised by synapse loss in the hippocampus and the inability to lay down new memories. Using our greater understanding of the types of NMDARs and AMPARs involved in LTP induction and expression respectively, it might be envisaged that the synapse could be modified to prevent the initial synaptotoxicity and consequent neurodegeneration at early stages of the disease, or adapted to restore plasticity to remaining synapses later in the condition. One way to modify synapses to this end may be the introduction of NMDARs with a higher potential for LTP. Alternatively, so called ‘designed receptors exclusively activated by designer drugs’ (DREADDs) could be used. This novel pharmacoge- netic technology involves stereotactic delivery of viral constructs containing DREADDs under specific promoter control so that engi-neered receptors are expressed by one type of neuron in a limited brain region, and then can be activated on demand by administration of a designer drug to stimulate their function, for example to enhance plasticity. It is also conceivable that downstream kinases could be targeted, but these may have more non-specific effects on cellular processes and so be less amenable to manipulation. Of course, the ability to restore learning without eradicating old memories is a huge challenge, but further study of the mechanisms that generate and maintain the aforementioned hippocampal asymmetry in the distribution of synapses with different propensities for plasticity could provide insight into how we may hope to achieve this in the future.

The possibility of genetically altering synaptic receptor composition in discrete brain regions has broader clinical ramifications than treating diseases ofsynaptic dysfunction.Theability to ‘reopen’a brain region for plasticity may be particularly useful in aiding recovery from stroke or brain injury. Moreover,since both optogenetics and DREADDs enable the stimulation or suppression of activity in specific neuronal populations and circuits, they might prove a desirable replacement for deep brain stimulation in the treatment of Parkinson’s disease and depression.They could also be developed to treat obsessive-compulsive disorder, addiction and otherwise intractable epilepsies. For example, it was recently shown that both optogenetic and K+ channel gene therapy were successful treatments in a rat model of focal neocortical epilepsy.27 Pharmacogenetics is a particularly appealing approach as, apart from the initial stereotactic surgery, it would be less invasive than chronic implantation of electrical or optical stimulation devices. Nevertheless, the ability to manipulate neuronal circuits at such a finely tuned level also brings with it many ethical considerations.

Conclusions

Huge advances have been made in the forty years since LTP was first formally described,though we are still unravelling the complexity of the various mechanisms of induction, expression and maintenance that support synaptic strengthening. The biggest scientific question in the field of whether or not LTP is the memory mechanism, remains unanswered. Excitingly, novel technologies now make it possible to test a causal relationship to definitively answer this question, and, moreover, probe how exactly plasticity supports learning and memory. Furthermore, there is potential scope to harness our current knowledge for therapeutic purposes, not only in disorders affecting learning and memory, but also to enhance recovery following brain injury.

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